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Catalog No: OCOA19426
Size:20UG
Price: $169.00
SKU
OCOA19426
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NONO Protein Lysate (OCOA19426)

Datasheets/ManualsPrintable datasheet for NONO Protein Lysate (OCOA19426)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionNONO HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolNONO
Gene Full Namenon-POU domain containing octamer binding
Alias Symbols54 kDa nuclear RNA- and DNA-binding protein;55 kDa nuclear protein;DNA-binding p52/p100 complex, 52 kDa subunit;MRXS34;NMT55;non-POU domain-containing octamer (ATGCAAAT) binding protein;non-POU domain-containing octamer-binding protein;NRB54;P54;p54(nrb);P54NRB;PPP1R114;protein phosphatase 1, regulatory subunit 114.
NCBI Gene Id4841
Protein NameA0A0S2Z4Z9, NM_001145408, NP_001138880, Q15233
Description of TargetHomo sapiens non-POU domain containing octamer binding (NONO), transcript variant 1, mRNA, Non-POU domain containing octamer-binding isoform 1, Non-POU domain-containing octamer-binding protein, non-POU domain-containing octamer-binding protein isoform 1 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q15233
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_001138880
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_001145408
Molecular Weight54.1 kDa
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