NONO Antibody - N-terminal region (ARP40715_T100)

Catalog No: ARP40715_T100

WB Suggested Anti-NONO Antibody Titration: 1.25ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate

Sample Type : subnuclear bodies-paraspeckles

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IF, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NONO
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: KQNHTPRKHHQHHHQQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLK
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-NONO (ARP40715_T100) antibody is Catalog # AAP40715 (Previous Catalog # AAPY00967)
• Reference: Kimura,K., (2006) Genome Res. 16 (1), 55-65
• Publications:

  Bubenik, J. L., Ladd, A. N., Gerber, C. A., Budiman, M. E. & Driscoll, D. M. Known turnover and translation regulatory RNA-binding proteins interact with the 3’ UTR of SECIS-binding protein 2. RNA Biol. 6, 73-83 (2009). 19106619

Target Info

• Gene Symbol: NONO
• Gene Full Name: Non-POU domain containing, octamer-binding
• Alias Symbols: P54, NMT55, NRB54, MRXS34, P54NRB, PPP1R114
• NCBI Gene Id: 4841
• Protein Name: Non-POU domain-containing octamer-binding protein
• Description of Target: NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
• Uniprot ID: Q15233
• Protein Accession #: NP_031389
• Nucleotide Accession #: NM_007363
• Protein Size (# AA): 471
• Molecular Weight: 52kDa
• Protein Interactions: SFPQ; PTBP1; PRKAA2; PIN1; NONO; FUS; DDX6; C11orf68; PSPC1; HUWE1; LMO4; UBC; SUMO2; STAU1; MDM2; RPA3; RPA2; RPA1; ASB18; WWOX; ASB3; ERG; EED; rev; WBP4; TCERG1; APBB1; PRPF40A; LMNA; CD81; PAN2; CTNNB1; ORC5; MYC; ITGA4; IL7R; FN1; EWSR1; YLPM1; RNF43