NOG High Sensitivity ELISA Kit (Human) (OKCD01371)

Catalog No: OKCD01371

Size:96WELLS

Human NOG ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human|Mouse|Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-101%	84-103%	89-96%	80-99%
  EDTA Plasma (n=5)	91-98%	78-103%	96-104%	97-104%
  Heparin Plasma (n=5)	80-104%	79-101%	87-104%	84-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Noggin (NOG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Noggin (NOG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Noggin (NOG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Noggin (NOG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92-103	98
  EDTA Plasma (n=5)	81-89	84
  Heparin Plasma (n=5)	98-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Noggin (NOG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Noggin (NOG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NOG Microplate: 96 Wells (12 x 8 Well Strips)
  • NOG Lyophilized Standard: 2
  • 100X Biotinylated NOG Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Noggin (NOG). No significant cross-reactivity or interference between Noggin (NOG) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NOG
• Gene Full Name: noggin
• Alias Symbols: noggin;SYM1;symphalangism 1 (proximal);SYNS1;SYNS1A.
• NCBI Gene Id: 9241
• Protein Name: Noggin
• Description of Target: Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed:21976273, PubMed:26643732).
• Uniprot ID: Q13253
• Protein Accession #: NP_005441.1
• Nucleotide Accession #: NM_005450.4