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Catalog No: OKCD02763
Size:96WELLS
Price: $700.00
SKU
OKCD02763
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Datasheets/ManualsPrintable datasheet for NNMT ELISA Kit (Mouse) (OKCD02763)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)84-103%87-95%80-89%78-101%
EDTA Plasma (n=5)92-101%85-102%96-103%81-96%
Heparin Plasma (n=5)87-94%94-102%86-95%88-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nicotinamide-N-Methyltransferase (NNMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nicotinamide-N-Methyltransferase (NNMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nicotinamide-N-Methyltransferase (NNMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nicotinamide-N-Methyltransferase (NNMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-10195
EDTA Plasma (n=5)95-10399
Heparin Plasma(n=5)80-9991
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinamide-N-Methyltransferase (NNMT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinamide-N-Methyltransferase (NNMT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NNMT Microplate96 Wells (12 x 8 Well strips)
NNMT Lyophilized Standard2
100X Biotinylated NNMT Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nicotinamide-N-Methyltransferase (NNMT). No significant cross-reactivity or interference between Nicotinamide-N-Methyltransferase (NNMT) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolNnmt
Gene Full Namenicotinamide N-methyltransferase
Alias Symbolsnicotinamide N-methyltransferase.
NCBI Gene Id18113
Protein NameNicotinamide N-methyltransferase
Description of TargetCatalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
Uniprot IDO55239
Protein Accession #NP_035054.1
Nucleotide Accession #NM_010924.3
Protein Size (# AA)264
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