Size:96 Wells
$649.00
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SKU
OKCD02715
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NME1 ELISA Kit (Rat) (OKCD02715)

Catalog#: OKCD02715
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Rat
Kit Detection Method Colorimetric, OD450 nm
Kit Duration 3 Hours
Kit Linearity
Sample1:21:41:81:16
Serum (n=5)99-105%80-89%84-91%81-98%
EDTA Plasma (n=5)85-93%78-91%90-104%93-101%
Heparin Plasma (n=5)94-101%87-95%88-104%91-103%
Kit Principle Aviva Systems Biology Nme1 ELISA Kit (Rat) (OKCD02715) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nme1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for Nme1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Nme1 captured in well.
Kit Range 0.312-20 ng/mL
Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)78-10385
EDTA Plasma (n=5)79-9185
Heparin Plasma(n=5)85-9590
Kit Reproducibility Intra-assay Precision: 3 samples with known low, middle and high level NME1 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level NME1 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
Kit Component
ComponentAmount
Anti-NME1 Microplate96 Wells (12 x 8 Well strips)
NME1 Lyophilized Standard2
100X Biotinylated NME1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma and other biological fluids
Sensitivity 0.115 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay has high sensitivity and excellent specificity for detection of Non Metastatic Cells 1, Protein NM23A Expressed In (NME1). No significant cross-reactivity or interference between Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) and analogues was observed.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol NME1
Official Gene Full Name NME/NM23 nucleoside diphosphate kinase 1
Alias Symbols Metastasis inhibition factor NM23, NDK A, NDP kinase A, Nucleoside diphosphate kinase A, Tumor metastatic process-associated protein
NCBI Gene Id 191575
Protein Name Nucleoside diphosphate kinase A
Description of Target Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Swissprot Id Q05982
Protein Accession # NP_612557.1
Nucleotide Accession # NM_138548.2
Protein Size (# AA) 152
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express NME1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express NME1.
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