NME1 ELISA Kit (Rat) (OKCD02715)

Catalog No: OKCD02715

Size:96WELLS

Rat NME1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	99-105%	80-89%	84-91%	81-98%
  EDTA Plasma (n=5)	85-93%	78-91%	90-104%	93-101%
  Heparin Plasma (n=5)	94-101%	87-95%	88-104%	91-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Non Metastatic Cells 1, Protein NM23A Expressed In (NME1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Non Metastatic Cells 1, Protein NM23A Expressed In (NME1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Non Metastatic Cells 1, Protein NM23A Expressed In (NME1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-103	85
  EDTA Plasma (n=5)	79-91	85
  Heparin Plasma(n=5)	85-95	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NME1 Microplate: 96 Wells (12 x 8 Well strips)
  • NME1 Lyophilized Standard: 2
  • 100X Biotinylated NME1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.115 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Non Metastatic Cells 1, Protein NM23A Expressed In (NME1). No significant cross-reactivity or interference between Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NME1
• Gene Full Name: NME/NM23 nucleoside diphosphate kinase 1
• Alias Symbols: Metastasis inhibition factor NM23, NDK A, NDP kinase A, Nucleoside diphosphate kinase A, Tumor metastatic process-associated protein
• NCBI Gene Id: 191575
• Protein Name: Nucleoside diphosphate kinase A
• Description of Target: Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
• Uniprot ID: Q05982
• Protein Accession #: NP_612557.1
• Nucleotide Accession #: NM_138548.2
• Protein Size (# AA): 152