NLRP1A ELISA Kit (Mouse) (OKWB00267)

Catalog No: OKWB00267

Size:96T

Mouse NLRP1A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	96-105%	85-103%	85-103%	93-105%
  EDTA Plasma (n=5)	84-99%	86-99%	82-97%	82-92%
  Heparin Plasma (n=5)	80-96%	84-99%	83-96%	90-99%

• ELISA Kit Principle: Aviva Systems Biology Nlrp1a ELISA Kit (Mouse) (OKWB00267) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nlrp1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nlrp1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nlrp1a captured in the well.
• ELISA Kit Range: 7.813-500pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	87-103	95
  EDTA Plasma (n=5)	92-101	96
  Heparin Plasma (n=5)	85-96	92

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Nlrp1a Microplate: 96 Wells (12 x 8 Well strips)
  • Nlrp1a Lyophilized Standard: 2 x 500 pg
  • 100X Nlrp1a Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 4.69 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Nlrp1a
• Gene Full Name: NLR family, pyrin domain containing 1A
• Alias Symbols: CARD7;caspase recruitment domain protein;caspase recruitment domain-containing protein 7;death effector filament-forming Ced-4-like apoptosis protein;DEFCAP;Gm14;Gm15;N;NAC;NACHT, leucine rich repeat and PYD containing 1;NACHT, LRR and PYD containing protein 1;NACHT, LRR and PYD domains-containing protein 1a;NACHT/LRR/pyrin domain-containing protein 1;Nalp1;Nalp1a;NLR family, pyrin domain containing 1;NLR family, pyrin domain containing 1A, CAST/EiJ strain specific;NLR family, pyrin domain containing 1A, PWK/PhJ strain specific;NLR family, pyrin domain containing 1A, WSB/EiJ strain specific;Nlrp1;nucleotide-binding domain and caspase recruitment domain;PP1044.
• NCBI Gene Id: 195046
• Protein Name: NACHT, LRR and PYD domains-containing protein 1a
• Description of Target: As a potential sensor component of the NLRP1 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of Nlrp1b, CASP1, and possibly PYCARD. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP1 inflammasome is also required for HMGB1 secretion. The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death (By similarity) (PubMed:23219391). When activated in the bone marrow, induces the pyroptosis of hematopoietic stem cells and progenitor cells of both myeloid and lymphoid lineages, hence allowing the removal of damaged cells, and the release of IL1B, which induces granulopoiesis (PubMed:23219391). Binds ATP (By similarity).
• Uniprot ID: Q2LKU9
• Protein Accession #: NP_001004142
• Nucleotide Accession #: NM_001004142