NLRC4 Antibody (OAPB00186)

Catalog No: OAPB00186 (Formerly GWB-4099E8)

Size:100UG

Western blot analysis of Ipaf in human PBL lysate with Ipaf antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) μg/mL, respectively.

Immunofluorescence of Ipaf in THP1 cells with Ipaf antibody at 10 ug/mL.

Immunocytochemistry of Ipaf in THP-1 cells with Ipaf antibody at 10 μg/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. Ipaf Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: Ipaf antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Ipaf antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf. The immunogen is located within the last 50 amino acids of Ipaf.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-NLRC4 (OAPB00186) antibody is Catalog # OEPB02282
• Application Info: Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 0.5 to 2 ug/ml. Human peripheral blood leukocyte (PBL) cell lysate can be used as positive control and a 110 kDa band can be detected. Ipaf antibody is human specific. (Optimal dilution should be determined by user)
• Reference: Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.;Poyet J-L, Srinivaula SM, Tnani M, et al. Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1. J. Biol. Chem. 2001; 276:28309-13.;Geddes BJ, Wang L, Huang WJ, et al. Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis. Biochem. Biophys. Res. Commun. 2001; 284:77-82.;

Target Info

• Gene Symbol: NLRC4
• Gene Full Name: NLR family CARD domain containing 4
• Alias Symbols: AIFEC;CARD, LRR, and NACHT-containing protein;CARD12;caspase recruitment domain family, member 12;caspase recruitment domain-containing protein 12;CED-4-like protein Clan;CLAN;CLAN1;CLANA;CLANB;CLANC;CLAND;CLR2.1;FCAS4;Ice protease-activating factor;ICE-protease activating factor;IPAF;NLR family CARD domain-containing protein 4;NOD-like receptor C4;nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4.
• NCBI Gene Id: 58484
• Protein Name: NLR family CARD domain-containing protein 4|NLR family CARD domain-containing protein 4 isoform a [Homo sapiens]
• Description of Target: Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis (PubMed:15107016). The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).
• Uniprot ID: Q9NPP4
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_067032
• Nucleotide Accession #: NM_001199138.1; NM_001199139.1; NM_001302504.1; NM_021209.4
• Molecular Weight: 110 kDa