NKX6-1 ELISA Kit (Human) (OKCD01010)

Catalog No: OKCD01010 (Formerly GWB-KBBHS7)

Size:96WELLS

Human NKX6-1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NK6 Homeobox Protein 1 (NKX6-1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NK6 Homeobox Protein 1 (NKX6-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NK6 Homeobox Protein 1 (NKX6-1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NK6 Homeobox Protein 1 (NKX6-1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NK6 Homeobox Protein 1 (NKX6-1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NK6 Homeobox Protein 1 (NKX6-1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NKX6-1 Microplate: 96 Wells (12 x 8 Well Strips)
  • NKX6-1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated NKX6-1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.131 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of NK6 Homeobox Protein 1 (NKX6-1). No significant cross-reactivity or interference between NK6 Homeobox Protein 1 (NKX6-1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NKX6-1
• Gene Full Name: NK6 homeobox 1
• Alias Symbols: homeobox protein NK-6 homolog A;homeobox protein Nkx-6.1;NK homeo box, family 6, member A;NK homeobox, family 6, A;NK6 transcription factor homolog A;NK6 transcription factor related, locus 1;NKX6.1;NKX6A.
• NCBI Gene Id: 4825
• Protein Name: Homeobox protein Nkx-6.1
• Description of Target: Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity).
• Uniprot ID: P78426
• Protein Accession #: NP_006159
• Nucleotide Accession #: NM_006168.2
• Protein Size (# AA): 367