Size:100 ul
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More Information
Predicted Species Reactivity Human, Mouse
Product Format Liquid. PBS containing 20% glycerol and 0.025% Proclin300.
Clonality Polyclonal
Isotype IgG
Host Rabbit
Application ICC, IF, WB
:: Cellular Localization: Nucleus
Reconstitution and Storage Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Replacement Item This antibody may replace item sc-12514 from Santa Cruz Biotechnology.
Immunogen Recombinant fragment corresponding to a region within amino acids 1 and 218 of Nkx2.5 (Uniprot ID#P52952)
Purification Purified by antigen-affinity chromatography.
Predicted Homology Based on Immunogen Sequence Rat, Dog, Bovine
Concentration 1 mg/ml
Datasheets/Manuals Printable datasheet for anti-NKX2-5 antibody - OAGA00423
Application Info WB 1:500~3000
ICC/IF 1:100~1000.
Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Gene Symbol NKX2-5
Official Gene Full Name Homeobox protein Nkx-2.5
Alias Symbols CHNG5 antibody, CSX antibody, CSX1 antibody, FLJ52202 antibody, FLJ97166 antibody, FLJ97195 antibody, FLJ97197 antibody, FLJ99536 antibody, NKX2.5 antibody, NKX2E antibody, NKX4-1 antibody, NKX2-5 antibody, tinman paralog antibody, homeobox protein Nkx-2.
NCBI Gene Id 1482
Protein Name Homeobox protein Nkx-2.5
Description of Target Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]
Swissprot Id P52952
Protein Accession # NP_001159647.1
Molecular Weight 35 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express NKX2-5.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express NKX2-5.
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