Catalog No: OCOA14095
Size:20UG
Price: $179.00
SKU
OCOA14095
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NKX2-2 Protein Lysate (OCOA14095)

Datasheets/ManualsPrintable datasheet for NKX2-2 Protein Lysate (OCOA14095)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionNKX2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolNKX2-2
Gene Full NameNK2 homeobox 2
Alias Symbolshomeobox protein NK-2 homolog B;homeobox protein Nkx-2.2;NK2 transcription factor related, locus 2;NK2 transcription factor-like protein B;NKX2.2;NKX2B.
NCBI Gene Id4821
Protein NameNM_002509, NP_002500, O95096
Description of TargetHomeobox protein Nkx-2.2, homeobox protein Nkx-2.2 [Homo sapiens], Homo sapiens NK2 homeobox 2 (NKX2-2), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/O95096
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_002500
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_002509
Molecular Weight30 kDa
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