NIX Antibody - middle region (OAAB08029)

Catalog No: OAAB08029

Size:400ul

NIX Antibody (Center) (Cat. #OAAB08029) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NIX antibody detected the NIX protein (arrow).

BNIP3L is strongly supported by BioGPS gene expression data to be expressed in A549

NIX Antibody (Center) (Cat. #OAAB08029)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NIX Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

NIX Antibody (Center) (Cat. #OAAB08029) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of NIX Antibody (Center)(Cat#OAAB08029) with A549 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human, Mouse, Cow
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, IF, FC
• Additional Information: Precautions: NIX Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor
  Cellular Location: Nucleus envelope. Endoplasmic reticulum. Mitochondrion outer membrane. Membrane; Single-pass membrane protein (Potential). Note=Colocalizes with SPATA18 at the mitochondrion outer membrane
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This NIX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-81 amino acids from the Central region of human NIX.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  BNIP3L is strongly supported by BioGPS gene expression data to be expressed in A549

• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: BNIP3L
• Alias Symbols: NIX, BNIP3a
• NCBI Gene Id: 665
• Protein Name: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O60238
• Protein Accession #: NP_004322.1
• Molecular Weight: 23930 Da