Catalog No: OKEH02600
Size:96T
Price: $600.00
SKU
OKEH02600
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Nitrotyrosine ELISA Kit (OKEH02600) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Nitrotyrosine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Nitrotyrosine and incubated. The Nitrotyrosine found in the sample or standards competes with the biotinylated Nitrotyrosine for limited binding sites on the immobilized anti-Nitrotyrosine antibody. Excess unbound biotinylated Nitrotyrosine and sample or standard Nitrotyrosine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Nitrotyrosine captured in the well and inversely proportional to the amount of Nitrotyrosine which was contained in the sample or standard.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 81%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 6.6% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Nitrotyrosine Microplate96 Wells (12 x 8 Well strips)
Nitrotyrosine Lyophilized Standard2 x 1,000 pg
Sample Diluent1 x 20 mL
100X Nitrotyrosine-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: NT
Target Name: Nitrotyrosine
::Pubchem: 65124
::Chemical formula: C9H10N2O5
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity4.9 pg/mL
SpecificityNatural and recombinant General Nitrotyrosine
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsNT, Nitrotyrosine, NT, 3-Nitrotyrosine, 3-Nitro-L-tyrosine, 3NT
Protein NameNitrotyrosine
Description of TargetNitrotyrosine is a product of tyrosine nitration mediated by reactive nitrogen species such as peroxynitrite anion and nitrogen dioxide. It is detected in large number of pathological conditions and is considered a marker of NO-dependent, reactive nitrogen species-induced nitrative stress. Nitrotyrosine is found in numerous disease-affected tissues, such as the cornea in keratoconus. Peroxynitrite and/or nitrative stress may participate in the pathogenesis of diabetes.
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