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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OASE00079-HRP |
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Predicted Species Reactivity | Human, Mouse, Rat, Dog |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Monoclonal |
Clone | 39B6 |
Isotype | IgG2a |
Host | Mouse |
Conjugation | HRP: Horseradish Peroxidase |
Application | WB, ELISA, IHC, IP, IF |
Additional Information | Background Info: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific. |
:: | Scientific Background: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4). |
:: | Certificate of Analysis: 0.7ug/ml was sufficient for detection of 5ug SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | Hybridoma line 39B6 |
Purification | Protein G Purified |
Concentration | 0.6-0.7 mg/ml |
Dilution | 0.7ug/ml was sufficient for detection of 5ug SIN-1 treated BSA by Western Blot |
Storage Buffer | PBS, 50% glycerol |
Reference | 1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387. 2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8. 3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30. 4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45. 5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199. 6. Khan J. et al. (1998) Biochem J. 330(2): 795-801. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Nitrotyrosine Antibody : HRP (OASE00079-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Dog".
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How long will it take to receive "Nitrotyrosine Antibody : HRP (OASE00079-HRP)"?
This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".
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What buffer format is "Nitrotyrosine Antibody : HRP (OASE00079-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Nitrotyrosine Antibody : HRP (OASE00079-HRP)"?
This target may also be called "" in publications.
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What is the shipping cost for "Nitrotyrosine Antibody : HRP (OASE00079-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Nitrotyrosine Antibody : HRP (OASE00079-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Nitrotyrosine Antibody : HRP (OASE00079-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Nitrotyrosine Antibody : HRP (OASE00079-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for ""?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with ""?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with ""?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with ""?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with ""?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.