Nitrotyrosine Antibody : FITC (OASE00079-FITC)

Catalog No: OASE00079-FITC

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Dog
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 39B6
• Isotype: IgG2a
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: WB, ELISA, IHC, IP, IF
• Additional Information: Background Info: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
  Scientific Background: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an
  important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase.
  Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis,
  Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).
  Certificate of Analysis: 0.7ug/ml was sufficient for detection of 5ug SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Hybridoma line 39B6
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 0.7ug/ml was sufficient for detection of 5ug SIN-1 treated BSA by Western Blot
• Storage Buffer: PBS, 50% glycerol
• Reference: 1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387.
  2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.
  3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30.
  4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45.
  5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199.
  6. Khan J. et al. (1998) Biochem J. 330(2): 795-801.