NISCH Recombinant Protein (OPCD05670)

Catalog No: OPCD05670

NISCH Recombinant Protein (OPCD05670) using SDS-PAGE

NISCH Recombinant Protein (OPCD05670) using SDS-PAGE

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
• Host: E.coli
• Conjugation: Unconjugated
• Application: Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot
• Additional Information: Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method)
  Residues: Leu1246-Ser1346
  Subcellular Location: Cell membrane. Cytoplasm. Early endosome. Recycling endosome.
• Reconstitution and Storage: 2°C to 8°C|-80°C
• Concentration: 200 ug/mL (prior to lyoph)
• Purity: > 95%
• Source: E.coli
• Tag: N-terminal His and GST Tag

Target Info

• Gene Symbol: NISCH
• Gene Full Name: nischarin
• Alias Symbols: hIRAS;I-1;I-1 receptor candidate protein;I1R candidate protein;imidazoline receptor 1;imidazoline receptor antisera selected;Imidazoline receptor antisera-selected protein;Imidazoline-1 receptor;Imidazoline-1 receptor candidate protein;IR1;IRAS;nischarin.
• NCBI Gene Id: 11188
• Protein Name: Nischarin
• Description of Target: Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways (By similarity). Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity (By similarity). Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation (By similarity). Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation (By similarity). Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells (By similarity). Inhibits lamellipodia formation, when overexpressed (By similarity). Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.
• Uniprot ID: Q9Y2I1
• Protein Accession #: NP_001263222.1
• Nucleotide Accession #: NM_001276293.1
• Protein Size (# AA): Leu1246~Ser1346
• Molecular Weight: 41.38kDa