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NIPBL Antibody (OAEB00848)

100 ug
$349.00
In Stock
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Gene Symbol:
NIPBL
NCBI Gene Id:
25836
Protein Name:
Transcriptional regulator EMBL DAA05331.1
Swissprot Id:
Q6IEH8
Protein Accession #:
NP_597677.2
Alias Symbols:
Goat Anti-NIPBL (aa922-935) Antibody, NIPBL, sister chromatid cohesion 2 homolog, SCC2 homolog, Scc2, nipped-B-like, Nipped-B homolog (Drosophila), IDN3-B, IDN3, FLJ44854, FLJ13648, FLJ13354, FLJ12597, FLJ11203, DKFZp434L1319, CDLS1, CDLS
Replacement Item:
This antibody may replace item sc-374625 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
ELISA, IHC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express NIPBL.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express NIPBL.
Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 92%
Complete computational species homology data:
NIPBL Antibody (OAEB00848)
Peptide Sequence:
RPDSPRVKQGDSNK
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Concentration:
100ug specific antibody in 200ul
Datasheets/Manuals:
Printable datasheet for anti-NIPBL antibody - OAEB00848
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: Antibody detection limit dilution 1:32000.
Immunohistochemistry: This product has been successfully used on paraffin embedded mouse tissues and on cryosections of mouse tissues and loss of signal was observed in Nipbl-deficient cells of homozygous mutant embryos.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 316kDa according to NP_597677.2 or 304kDa according to NP_056199.2. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring product.
Additional Information:
Epitope Info: internal region
Immunogen: Peptide with sequence C-RPDSPRVKQGDSNK, from the internal region of the protein sequence according to NP_597677.2; NP_056199.2.
:::
Note: This antibody is expected to recognize both reported isoforms (NP_597677.2; NP_056199.2).
Reactivity Info: Tested: Mouse; Expected: Human, Mouse, Rat, Dog, Cow

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