NIP1 Antibody (BH3 Domain Specific) (OAAB09461)

Catalog No: OAAB09461

Size:400ul

Western blot analysis of anti-NIP1 BH3 Domain Pab (Cat.# AP1335a) in HL60 cell lysates (35ug/lane). NIP1 BH3 Domain (arrow) was detected using the purified Pab.

BNIP1 is strongly supported by BioGPS gene expression data to be expressed in HL60

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: NIP1 Antibody (BH3 Domain Specific) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death. May be involved in mitochondrial autophagy
  Cellular Location: Mitochondrion. Endoplasmic reticulum membrane; Single-pass type IV membrane protein
  Tissue Location: Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This NIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-127 amino acids from human NIP1.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  BNIP1 is strongly supported by BioGPS gene expression data to be expressed in HL60

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: BNIP1
• Alias Symbols: NIP1, SEC20, TRG-8
• NCBI Gene Id: 662
• Protein Name: Vesicle transport protein SEC20
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q12981
• Protein Accession #: NP_001196.2
• Molecular Weight: 26132 Da