NHP2L1 Antibody (OAGA00751)

Catalog No: OAGA00751

Size:100 ul

Sample (30 ug of whole cell lysate)
A: HeLa
B: Hep G2
12% SDS PAGE
OAGA00751 diluted at 1:1000

NHP2L1 antibody detects NHP2L1 protein by Western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
D. 30 ug NIH-3T3 whole cell lysate/extract
E. 30 ug BCL-1 whole cell lysate/extract
F. 30 ug Raw264.7 whole cell lysate/extract
G. 30 ug C2C12 whole cell lysate/extract
15 % SDS-PAGE
NHP2L1 antibody (OAGA00751) dilution: 1:1000

NHP2L1 antibody detects NHP2L1 protein by Western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
15 % SDS-PAGE
NHP2L1 antibody (OAGA00751) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NHP2-like protein 1(OAGA00751) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NHP2-like protein 1(OAGA00751) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
  Positive Controls: 293T, H1299, HeLa, HepG2, Molt-4, Raji
  Cellular Localization: Nucleus, nucleolus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 128 of NHP2-like protein 1 (Uniprot ID#P55769)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: NHP2L1
• Gene Full Name: NHP2-like protein 1
• Alias Symbols: FA1, FA-1, NHPX, 15.5K, OTK27, SSFA1, NHP2L1, SPAG12, SNRNP15-5
• NCBI Gene Id: 4809
• Protein Name: NHP2-like protein 1
• Uniprot ID: P55769
• Protein Accession #: NP_001003796.1
• Molecular Weight: 14 kDa