Nhe-1 Antibody (OAPB00594)

Catalog No: OAPB00594

Size:100UG

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Western blot analysis of Nhe-1 in rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 μg/mL.

Immunohistochemical staining of human brain tissue using Nhe-1 antibody at 2.5 μg/mL.

Immunofluorescence of Nhe-1 in Human Brain cells with Nhe-1 antibody at 20 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Porcine|Rabbit|Rat
• Product Format: Liquid. Nhe-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: Nhe-1 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Nhe-1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Nhe-1. The immunogen is located within the last 50 amino acids of Nhe-1.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-Nhe-1 antibody is Catalog # OEPB02710
• Application Info: Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Mendoza SA. The Na+-H+ antiport is a mediator of cell proliferation. Acta Paediatr. Scand.1987; 76:545-7.;Denker SP, Huang DC, Orlowski J, et al. Direct binding of the NA—H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation. Mol. Cell.2000; 6:1425-36.;Cingolani HE, Rebolledo OR, Portiansky EL, et al. Regression of hypertensive myocardial fibrosis by NA (+)/H(+) exchange inhibition. Hypertension2003; 41:373-7.;

Target Info

• Gene Symbol: SLC9A1
• Gene Full Name: solute carrier family 9 member A1
• Alias Symbols: APNH;LIKNS;Na(+)/H(+) antiporter, amiloride-sensitive;Na(+)/H(+) exchanger 1;Na-Li countertransporter;NHE1;NHE-1;PPP1R143;protein phosphatase 1, regulatory subunit 143;sodium/hydrogen exchanger 1;solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive);Solute carrier family 9 member 1;solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1.
• NCBI Gene Id: 6548
• Protein Name: Sodium/hydrogen exchanger 1
• Description of Target: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
• Uniprot ID: P19634
• Molecular Weight: Predicted: 52, 90 kDa Observed: 50, 90 kDa