Nhe-1 Antibody : HRP (OAPB00594-HRP)

Catalog No: OAPB00594-HRP

Size:0.1 mg

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Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: ELISA, WB, IHC
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen for anti-Nhe-1 antibody: antibody was raised against a 20 amino acid peptide near the carboxy terminus of the human Nhe-1.
• Purification: Affinity chromatography purified via peptide column
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 0.6-0.7 mg/ml
• Blocking Peptide: For anti-Nhe-1 antibody is Catalog # OEPB02710
• Application Info: Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Mendoza SA. The Na+-H+ antiport is a mediator of cell proliferation. Acta Paediatr. Scand.1987; 76:545-7.;Denker SP, Huang DC, Orlowski J, et al. Direct binding of the NA—H exchanger NHE1 to ERM proteins regulates the cortical cytoskeleton and cell shape independently of H(+) translocation. Mol. Cell.2000; 6:1425-36.;Cingolani HE, Rebolledo OR, Portiansky EL, et al. Regression of hypertensive myocardial fibrosis by NA (+)/H(+) exchange inhibition. Hypertension2003; 41:373-7.;

Target Info

• Gene Symbol: SLC9A1
• Alias Symbols: APNH, NHE1, LIKNS, NHE-1, PPP1R143
• NCBI Gene Id: 6548
• Protein Name: Sodium/hydrogen exchanger 1
• Description of Target: (NT) Sodium-hydrogen exchanger 1
• Uniprot ID: P19634