NGFRAP1 Antibody (OAPB00250)

Catalog No: OAPB00250 (Formerly GWB-36DCE7)

Size:100UG

Click here to learn more about Aviva's By-Request Conjugation Service.

Western blot analysis of NADE in Human brain cell lysates with NADE antibody at 1 ug/mL in the presence (A) or absence (B) of blocking peptide.

Immunohistochemistry of NADE in human brain tissue with NADE antibody at 2 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Liquid. NADE Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: NADE antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: NADE antibody was raised against a peptide corresponding to 14 amino acids near the middle of human NADE. The immunogen is located within the last 50 amino acids of NADE.
• Purification: Ion Exchange
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-NGFRAP1 (OAPB00250) antibody is Catalog # OEPB02343
• Application Info: NADE antibody can be used for detection of NADE by Western blot at 0.5 to 2 ug/ml. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE. (Optimal dilution should be determined by user)
• Reference: Gentry JJ, Barker PA, and Cater BD. The p75 neurotrophin receptor: multiple interactors and numerous functions. Pro. Brain Res. 2004; 146:25-39.;Mukai J, Hachiya T, Shoji-Hoshino S, et al. NADE, a p75NTR-associated cell death executor, is involved in signal transduction mediated by the common neurotrophin receptor p75NTR. J. Biol. Chem. 2000; 275:17566-70.;Rapp G, Freudenstein J, Klaudiny J, et al. Characterization of three abundant mRNAs from human ovarian granulosa cells. DNA Cell Biol. 1990; 9:479-85.;YI J-S, Lee, S-K, Sato T-A, et al. Co-induction of p75NTR and the associated death executor NADE in degenerating hippocampal neurons after kainate-induced seizures in the rat. Neurosci. Lett. 2003; 347:126-30.

Target Info

• Gene Symbol: BEX3
• Gene Full Name: brain expressed X-linked 3
• Alias Symbols: Bex;brain-expressed X-linked protein 3;DXS6984E;HGR74;NADE;nerve growth factor receptor (TNFRSF16) associated protein 1;Nerve growth factor receptor-associated protein 1;NGFRAP1;ovarian granulosa cell 13.0 kDa protein HGR74;p75NTR-associated cell death executor;protein BEX3.
• NCBI Gene Id: 27018
• Protein Name: Protein BEX3|protein BEX3 isoform b [Homo sapiens]
• Description of Target: May be a signaling adapter molecule involved in p75NTR-mediated apoptosis induced by NGF. Plays a role in zinc-triggered neuronal death (By similarity). May play an important role in the pathogenesis of neurogenetic diseases.
• Uniprot ID: Q00994
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_996798
• Nucleotide Accession #: NM_014380.2; NM_206915.2; NM_206917.2