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Catalog No: OKIA00031 (Formerly GWB-374Z23)
Size:1x96WELLPLATE
Price: $577.00
SKU
OKIA00031
Availability: Domestic: within 1 week delivery | International: 1 week
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityDog
Product FormatIn Solution
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration210 Minutes
ELISA Kit PrincipleAviva Systems Biology NGAL ELISA Kit (Dog) (OKIA00031) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for NGAL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-NGAL - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NGAL captured in the well.
ELISA Kit Range15.6 pg/ml - 1000 pg/ml
ELISA Kit Component
ComponentAmount
Anti-Dog NGAL (Lipocalin-2) Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Dog NGAL (Lipocalin-2) Calibrator1
Additional InformationIntended Use: The NGAL test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring NGAL in biological samples of Dogs.
::Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Dog NGAL antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in
citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-DOG NGAL ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Dog NGAL.
7. DOG NGAL CALIBRATOR
One vial containing Dog NGAL calibrator.
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.010 ng/ml
Predicted Homology Based on Immunogen SequenceDog
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Alias SymbolsLipocalin 2, Neutrophil gelatinase-associated lipocalin, NGAL
Protein NameNGAL
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