NFYC ELISA Kit (Human) (OKCD00981)

Catalog No: OKCD00981 (Formerly GWB-KBBJO6)

Size:96WELLS

Human NFYC ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Transcription Factor Y Gamma (NFYC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Transcription Factor Y Gamma (NFYC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Transcription Factor Y Gamma (NFYC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nuclear Transcription Factor Y Gamma (NFYC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Transcription Factor Y Gamma (NFYC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Transcription Factor Y Gamma (NFYC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NFYC Microplate: 96 Wells (12 x 8 Well Strips)
  • NFYC Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated NFYC Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.114 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nuclear Transcription Factor Y Gamma (NFYC). No significant cross-reactivity or interference between Nuclear Transcription Factor Y Gamma (NFYC) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NFYC
• Gene Full Name: nuclear transcription factor Y subunit gamma
• Alias Symbols: CAAT box DNA-binding protein subunit C;CBFC;CBF-C;CCAAT binding factor subunit C;CCAAT transcription binding factor subunit gamma;H1TF2A;HAP5;histone H1 transcription factor large subunit 2A;HSM;NF-YC;nuclear transcription factor Y subunit C;nuclear transcription factor Y subunit gamma;nuclear transcription factor Y, gamma;transactivator HSM-1;transactivator HSM-1/2;transcription factor NF-Y, C subunit.
• NCBI Gene Id: 4802
• Protein Name: Nuclear transcription factor Y subunit gamma
• Description of Target: Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
• Uniprot ID: Q13952
• Protein Accession #: NP_001136059
• Nucleotide Accession #: NM_001142587.1
• Protein Size (# AA): 458