NFX1 Antibody - middle region (P100680_P050)

Catalog No: P100680_P050

WB Suggested Anti-NFX1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate

Immunohistochemistry with Human Testis lysate tissue

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFX1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 85%; Dog: 85%; Human: 100%; Mouse: 76%; Rabbit: 85%; Rat: 85%
• Peptide Sequence: Synthetic peptide located within the following region: TLTGVLEREMQARPPPPIPHHRHQSDKNPGSSNLQKITKEPIIDYFDVQD
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-NFX1 (P100680_P050) antibody is Catalog # AAP31019 (Previous Catalog # AAPP01752)
• Reference: Katzenellenbogen,R.A., (2007) J. Virol. 81 (8), 3786-3796

Target Info

• Gene Symbol: NFX1
• Gene Full Name: Nuclear transcription factor, X-box binding 1
• Alias Symbols: NFX2, Tex42, TEG-42
• NCBI Gene Id: 4799
• Protein Name: Transcriptional repressor NF-X1
• Description of Target: MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.
• Uniprot ID: Q12986
• Protein Accession #: NP_002495
• Nucleotide Accession #: NM_002504
• Protein Size (# AA): 1120
• Molecular Weight: 124kDa
• Protein Interactions: UBC; BMI1; HECW2; SRSF4; SRSF3; RPS19; RPS3A; RPL22; RPL17; RPL13; RPL7A; RPL6; RPL3; PABPC3; YBX1; NCL; HNRNPM; HSPA1A; HNRNPU; HNRNPH1; HNRNPA2B1; HIST1H2AE; EEF1A2; DMD; DDX5; FAM208B; PABPC1; RPL13A; LARP1; APC2; MATR3; HIST1H4E; USP9X; SIN3A; MAML3;