Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OCOA00806
Size:20UG
Price: $275.00
SKU
OCOA00806
Availability: Domestic: within 1 week delivery | International: 1 week
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

NFKB2 Protein Lysate (OCOA00806)

Datasheets/ManualsPrintable datasheet for NFKB2 Protein Lysate (OCOA00806)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionNFKB2 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolNFKB2
Gene Full Namenuclear factor kappa B subunit 2
Alias SymbolsCVID10;DNA-binding factor KBF2;H2TF1;lymphocyte translocation chromosome 10 protein;LYT10;LYT-10;NFKB, p52/p100 subunit;NF-kB2;nuclear factor Kappa-B, subunit 2;nuclear factor NF-kappa-B p100 subunit;nuclear factor NF-kappa-B p52 subunit;nuclear factor of Kappa light chain gene enhancer in B cells 2;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2;nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100);oncogene Lyt-10;p100;p49/p100;p52;transcription factor NFKB2.
NCBI Gene Id4791
Protein NameNM_002502, NP_002493, Q00653
Description of TargetHomo sapiens nuclear factor kappa B subunit 2 (NFKB2), transcript variant 2, mRNA, Nuclear factor NF-kappa-B p100 subunit, nuclear factor NF-kappa-B p100 subunit isoform b [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q00653
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_002493
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_002502
Molecular Weight96.5 kDa
Write Your Own Review
You're reviewing:NFKB2 Protein Lysate (OCOA00806)
Your Rating