NFKB1 ELISA Kit (Dog) (OKEH08433)

Catalog No: OKEH08433

Size:96T

Dog NFKB1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	87-97% (n = 20)	90-99% (n = 20)	88-98% (n = 20)	95-104% (n = 20)
  EDTA plasma(n=5)	106-114% (n = 20)	101-110% (n = 20)	105-118% (n = 20)	92-101% (n = 20)
  heparin plasma(n=5)	98-108% (n = 20)	97-106% (n = 20)	100-112% (n = 20)	101-109% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology NFKB1 ELISA Kit (Dog) (OKEH08433) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NFKB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NFKB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NFKB1 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.7% (n = 20)
  Mean Inter-assay CV%: <=6.2% (n = 20)
• ELISA Kit Component:
  • NFKB1 Microplate: 96 Wells (12 x 8 Well strips)
  • NFKB1 Lyophilized Standard: 2
  • 100X Biotinylated NFKB1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
  Subcellular Location: Nucleus Cytoplasm Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.08ng/mL
• Specificity: Natural and recombinant Dog Nuclear factor NF-kappa-B p105 subunit
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NFKB1
• Gene Full Name: nuclear factor kappa B subunit 1
• Alias Symbols: nuclear factor NF-kappa-B p105 subunit;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1;nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105).
• NCBI Gene Id: 442859
• Protein Name: Q6F3J0
• Description of Target: Nuclear factor NF-kappa-B p105 subunit
• Uniprot ID: https://www.uniprot.org/uniprot/Q6F3J0
• Protein Accession #: NP_001003344.1
• Nucleotide Accession #: NM_001003344.1