Catalog No: OAAF00642 (Formerly GWB-ASC052)
Size:100 ug
Price: $344.00
SKU
OAAF00642
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for NFKB1 Antibody (OAAF00642)
Product Info
Predicted Species ReactivityHuman|Mouse
Product FormatLiquid
ClonalityPolyclonal
HostRabbit
ApplicationEnzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Reconstitution and Storage-20°C
ImmunogenThe antiserum was produced against synthesized peptide derived from human NF-kappaB p105/p50.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
SpecificityNF-κB p105/p50 (Ab-927) antibody detects endogenous levels of total NF-κB p105/p50 protein.
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
DilutionThe optimal working dilution should be determined by the end user.
Application InfoWB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:40000
Referenceohn J. Haddad, J. Pharmacol. Exp. Ther., Feb 2002; 300: 567.
Sabine Kirchhoff, Eur. J. Biochem., Apr 1999; 261: 546.
Gerald Thiel, Eur. J. Biochem., Jul 2004; 271: 2855 – 2862.
Gene SymbolNFKB1
Gene Full Namenuclear factor kappa B subunit 1
Alias SymbolsCVID12;DNA-binding factor KBF1;EBP-1;KBF1;NFkappaB;NF-kappaB;NF-kappa-B1;NF-kappabeta;NF-kB;NF-kB1;NFKB-p105;NFKB-p50;nuclear factor kappa-B DNA binding subunit;nuclear factor NF-kappa-B p105 subunit;nuclear factor NF-kappa-B p50 subunit;nuclear factor of kappa light polypeptide gene enhancer in B-cells 1.
NCBI Gene Id4790
Protein NameNuclear factor NF-kappa-B p105 subunit
Description of TargetNF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.
Uniprot IDP19838
Protein Accession #NP_001158884.1
Molecular Weight105 kDa
  1. What is the species homology for "NFKB1 Antibody (OAAF00642)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".

  2. How long will it take to receive "NFKB1 Antibody (OAAF00642)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "NFKB1 Antibody (OAAF00642)" provided in?

    This item is provided in "Liquid".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "NFKB1 Antibody (OAAF00642)"?

    This target may also be called "CVID12;DNA-binding factor KBF1;EBP-1;KBF1;NFkappaB;NF-kappaB;NF-kappa-B1;NF-kappabeta;NF-kB;NF-kB1;NFKB-p105;NFKB-p50;nuclear factor kappa-B DNA binding subunit;nuclear factor NF-kappa-B p105 subunit;nuclear factor NF-kappa-B p50 subunit;nuclear factor of kappa light polypeptide gene enhancer in B-cells 1." in publications.

  5. What is the shipping cost for "NFKB1 Antibody (OAAF00642)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "NFKB1 Antibody (OAAF00642)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "NFKB1 Antibody (OAAF00642)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "105 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "NFKB1 Antibody (OAAF00642)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "NFKB1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "NFKB1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "NFKB1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "NFKB1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "NFKB1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "NFKB1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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