NFKB1 Antibody (OAAD00076)

Catalog No: OAAD00076

Size:0.5 ml

Product Info

• Predicted Species Reactivity: Human
• Product Format: Concentrated liquid. Supplied in 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5.
• Clonality: Monoclonal
• Clone: B301
• Isotype: IgM
• Host: Mouse
• Application: WB, IHC
• Additional Information: Recommended Positive Control: Human tonsil or lymph node.
  
• Reconstitution and Storage: Aliquoting Instructions: Do not dilute the entire reconsituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. GENERAL INFO: When diluting for immunohistochemistry, ELISA or western blot, make the dilution in Antibody Diluting Buffer. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
  Store at 2 - 8C for short term, freeze under -20C for long term storage.
• Immunogen: Activated peripheral blood nuclei.
• Specificity: The p105 protein, found in all human cells in a dimeric form (105 kD) and monomeric form (41 kD), is a proliferation-associated nuclear antigen that is absent in non-cycling cells in the G0 phase. This protein is important in the synthesis and transport of RNA in the cell regulation cycle. During G2 and mitosis, this protein is increased possibly due to the unmasking of nucleolar antigens, resulting in large accumulations of this protein in the mitotic cytoplasm.
• Application Info: Immunohistochemistry -- 1:50-1:100
  
  Staining Procedure: This antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. FFPE tissue sections require pepsin or trypsin digestion. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.
  Western Blotting --1:100-1:200 .
• Reference: 1. Clevenger, CV., Epstein, AL., Exp.Cell.Res., 151:194-207, 1984.
  2. Clevenger, CV., Epstein, AL., J. of Histochemistry and Cytochemistry, 32: 757-765, 1984.
  3. Clevenger, CV., et. al., Cytometry 6:208-214, 1985.

Target Info

• Gene Symbol: NFKB1
• Gene Full Name: Nuclear factor NF-kappa-B p105 subunit
• Alias Symbols: KBF1, EBP-1, NF-kB, CVID12, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B1, NF-kappabeta
• NCBI Gene Id: 4790
• Protein Name: Nuclear factor NF-kappa-B p105 subunit
• Description of Target: Monoclonal Mouse Anti-Human p105 Proliferating-Associated Nuclear Antigen
• Uniprot ID: P19838
• Protein Accession #: NP_001158884.1; NP_003989.2
• Nucleotide Accession #: NM_001165412.1; NM_003998.3
• Molecular Weight: Dimeric form 105 kD, Monomeric form 41 kD