NFE2L2 ELISA Kit (Human) (OKCD02754)

Catalog No: OKCD02754

Size:96WELLS

Human NFE2L2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NFE2L2 Microplate: 96 Wells (12 x 8 Well strips)
  • NFE2L2 Lyophilized Standard: 2
  • 100X Biotinylated NFE2L2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.054 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). No significant cross-reactivity or interference between Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) and analogues was observed.
• Publications:

  Intermittent Hypoxia and Its Impact on Nrf2/HIF-1α Expression and ABC Transporters: An in Vitro Human Blood-Brain Barrier Model Study. Cell Physiol Biochem. 54, 1231-1248 (2020). 33326735

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for NFE2L2 ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: NFE2L2
• Gene Full Name: nuclear factor, erythroid 2 like 2
• Alias Symbols: HEBP1;IMDDHH;Nrf-2;NRF2;nuclear factor erythroid 2-related factor 2;nuclear factor erythroid-derived 2-like 2;Nuclear factor, erythroid derived 2, like 2.
• NCBI Gene Id: 4780
• Protein Name: Nuclear factor erythroid 2-related factor 2
• Description of Target: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
• Uniprot ID: Q16236
• Protein Accession #: NP_001138884.1
• Nucleotide Accession #: NM_001145412.3
• Protein Size (# AA): 605