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Catalog No: OKCD00210 (Formerly GWB-KBBNE7)
Size:96WELLS
Price: $750.00
SKU
OKCD00210
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Datasheets/ManualsPrintable datasheet for NFATC2 ELISA Kit (Human) (OKCD00210)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)93-101%78-90%91-101%94-104%
EDTA plasma(n=5)86-101%95-103%80-95%86-101%
heparin plasma(n=5)86-94%84-102%83-91%94-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)89-9893
EDTA plasma(n=5)93-10197
heparin plasma(n=5)92-10197
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NFATC2 Microplate96 Wells (12 x 8 Well Strips)
NFATC2 Lyophilized Standard2 x 1 ng
100X Biotinylated NFATC2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
Sensitivity< 5.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2). No significant cross-reactivity or interference between Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNFATC2
Gene Full Namenuclear factor of activated T cells 2
Alias SymbolsNFAT pre-existing subunit;NFAT transcription complex, preexisting component;NFAT1;NF-ATc2;NFATP;nuclear factor of activated T-cells, cytoplasmic 2;nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2;nuclear factor of activated T-cells, preexisting component;preexisting nuclear factor of activated T-cells 2;T cell transcription factor NFAT1;T-cell transcription factor NFAT1.
NCBI Gene Id4773
Protein NameNuclear factor of activated T-cells, cytoplasmic 2
Description of TargetPlays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.
Uniprot IDQ13469
Protein Accession #NP_001129493
Nucleotide Accession #NM_001136021.2
Protein Size (# AA)925
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