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Datasheets/Manuals | Printable datasheet for anti-NFATC1 (OASG04965) antibody |
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Predicted Species Reactivity | Human, Mouse |
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Product Format | Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | IHC, WB, ELISA |
Additional Information | Cellular Localization: Cytoplasm Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription . Nuclear translocation of NFATC1 is enhanced in the presence of TNFSF11. Nuclear translocation is decreased in the presence of FBN1 which can bind and sequester TNFSF11. Sequence and Domain Family: Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.; The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.; Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor. |
Reconstitution and Storage | Store at -20°C. Avoid repeated freeze/thaw cycles. |
Immunogen | Synthesized peptide derived between 230-310aa from human NFATc1 around the phosphorylation site of S294. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration | 1 mg/ml |
Target Post-Translational Modification | Phospho-Ser294; Phosphorylated by NFATC-kinase and GSK3B; phosphorylation induces NFATC1 nuclear exit and dephosphorylation by calcineurin promotes nuclear import. Phosphorylation by PKA and DYRK2 negatively modulates nuclear accumulation, and promotes subsequent phosphorylation by GSK3B or casein kinase 1. |
Specificity | Phospho-NFATc1 (S294) Polyclonal Antibody detects endogenous levels of NFATc1 protein only when phosphorylated at S294. |
Application Info | IHC: 1:100-1:300 ELISA: 1:10000 WB: 1:500~2000 |
Gene Symbol | NFATC1 |
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Gene Full Name | nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 |
Alias Symbols | NFAT2, NFATc, NF-ATC, NF-ATc1.2 |
NCBI Gene Id | 4772; |
Description of Target | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells . Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function. |
Uniprot ID | O95644, O88942 |
Molecular Weight | 105 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "NFATC1 Antibody (Phospho-Ser294) (OASG04965)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
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How long will it take to receive "NFATC1 Antibody (Phospho-Ser294) (OASG04965)"?
This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".
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What buffer format is "NFATC1 Antibody (Phospho-Ser294) (OASG04965)" provided in?
This item is provided in "Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "NFATC1 Antibody (Phospho-Ser294) (OASG04965)"?
This target may also be called "NFAT2, NFATc, NF-ATC, NF-ATc1.2" in publications.
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What is the shipping cost for "NFATC1 Antibody (Phospho-Ser294) (OASG04965)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "NFATC1 Antibody (Phospho-Ser294) (OASG04965)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "NFATC1 Antibody (Phospho-Ser294) (OASG04965)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "105 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "NFATC1 Antibody (Phospho-Ser294) (OASG04965)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "NFATC1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "NFATC1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "NFATC1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "NFATC1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "NFATC1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "NFATC1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.