Neuroligin 3 Antibody : Biotin (OASE00317-Biotin)

Catalog No: OASE00317-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: S110-29
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: WB, ICC, IHC
• Additional Information: Background Info: Detects a 110 kDa protein. Does not cross-react with Neuroligin-1, -2, -4 or -4.
  Scientific Background: Neuroligins are Type I membrane proteins enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. They have been characterized as neuronal cell surface proteins and are thought to be involved in cell-cell-interactions by forming intercellular junctions through binding to beta-neurexins. They play a major role in the formation or maintenance of synaptic junctions. They are also thought to be involved in the specification of excitatory synapses. Neuroligins interact with beta-neurexins and this interaction is involved in the formation of functional synapses.
  Certificate of Analysis: 1ug/mL was sufficient for detection of Neuroligin 3 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein amino acids 730-848 (intracellular C-terminus) of rat Neuroligin-3 (Accession no AAA97871)
  Mouse: 99% identity (118/119 amino acids identical)
  Human: 98% identity (116/119 amino acids identical)
  ~60% identity with Neuroligin-1
  ~40% identity with Neuroligin-2
  
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 WB
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide

Target Info

• Gene Symbol: Nlgn3
• Gene Full Name: neuroligin 3
• NCBI Gene Id: 171297
• Uniprot ID: Q62889
• Protein Accession #: AAA97871