Catalog No: OKCD00425 (Formerly GWB-KBBBN3)
Size:96WELLS
Price: $725.00
SKU
OKCD00425
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for NEU2 ELISA Kit (Rat) (OKCD00425)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)80-95%78-92%93-105%80-101%
EDTA plasma(n=5)87-94%78-101%97-105%80-98%
heparin plasma(n=5)84-97%90-102%93-101%87-99%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sialidase 2, Cytosolic (SIAL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sialidase 2, Cytosolic (SIAL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sialidase 2, Cytosolic (SIAL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sialidase 2, Cytosolic (SIAL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)97-105101
EDTA plasma(n=5)92-10396
heparin plasma(n=5)87-9491
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sialidase 2, Cytosolic (SIAL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sialidase 2, Cytosolic (SIAL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Neu2 Microplate96 Wells (12 x 8 Well Strips)
Neu2 Lyophilized Standard2 x 10 ng
100X Biotinylated Neu2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Sialidase 2, Cytosolic (SIAL2). No significant cross-reactivity or interference between Sialidase 2, Cytosolic (SIAL2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNEU2
Gene Full Nameneuraminidase 2
Alias SymbolsCytosolic sialidase;N-acetyl-alpha-neuraminidase 2.
NCBI Gene Id29204
Protein NameSialidase-2
Description of TargetCatalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins, oligosaccharides and gangliosides.
Uniprot IDQ64627
Protein Accession #NP_058826
Nucleotide Accession #NM_017130.2
Protein Size (# AA)379
Write Your Own Review
You're reviewing:NEU2 ELISA Kit (Rat) (OKCD00425)
Your Rating
We found other products you might like!