NES Recombinant Protein (Human) (OPCA04114)

Catalog No: OPCA04114

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Additional Information: Species Specificity Detail: Homo sapiens (Human)
• Reconstitution and Storage: Briefly centrifuge lyophilized product prior to opening to bring the contents to the bottom. Please reconstitute protein to 0.1-1.0 mg/mL by adding deionized sterile water first, followed by addition of glycerol to a final concentration of 5-50%. Reconstituted product should be aliquoted for long-term storage at -20C/-80C. Our in house default final concentration of glycerol is 50% for reference. The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
• Formulation: Tris-base, 50% glycerol
• Purity: Greater than 90% as determined by SDS-PAGE.
• Protein Sequence: Partial: PPPQGETGKEGWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLPGVPGEVAEPLGQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPL
• Storage Buffer: Tris-base, 50% glycerol
• Source: Yeast
• Tag: N-terminal 6xHis-tagged
• Reference: Characterization of the human nestin gene reveals a close evolutionary relationship to neurofilaments.Dahlstrand J., McKay R.D.G., Zimmerman L.B., Lendahl U.J. Cell Sci. 103:589-597(1992)

Target Info

• Gene Symbol: NES
• Gene Full Name: nestin
• Alias Symbols: Nbla00170
• NCBI Gene Id: 10763
• Protein Name: Nestin
• Description of Target: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells
• Uniprot ID: P48681
• Protein Accession #: NP_006608
• Nucleotide Accession #: NM_006617
• Molecular Weight: 28 kDa