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Catalog No: OKCD02752
Size:96WELLS
Price: $625.00
SKU
OKCD02752
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Datasheets/ManualsPrintable datasheet for NES ELISA Kit (Mouse) (OKCD02752)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nestin (NES). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nestin (NES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nestin (NES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nestin (NES) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nestin (NES) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nestin (NES) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NES Microplate96 Wells (12 x 8 Well strips)
NES Lyophilized Standard2
100X Biotinylated NES Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.055 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nestin (NES). No significant cross-reactivity or interference between Nestin (NES) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolNes
Gene Full Namenestin
Alias SymbolsAA166324;C78523;ESTM4;ESTM46;Ifa;Ifaprc2;intermediate filament protein;intermediate filament-associated protein RC2;Mar;Marc2;nestin;RC;RC2.
NCBI Gene Id18008
Protein NameNestin
Description of TargetRequired for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division (By similarity). Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Uniprot IDQ6P5H2
Protein Accession #NP_057910.3
Nucleotide Accession #NM_016701.3
Protein Size (# AA)1864
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