Catalog No: OKCD01073 (Formerly GWB-KBBTM4)
Size:96WELLS
Price: $750.00
SKU
OKCD01073
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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NELL2 ELISA Kit (Human) (OKCD01073)
Datasheets/Manuals | Printable datasheet for NELL2 ELISA Kit (Human) (OKCD01073) |
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COA Datasheet | ClickHereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEL Like Protein 2 (NELL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NEL Like Protein 2 (NELL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEL Like Protein 2 (NELL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NEL Like Protein 2 (NELL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NEL Like Protein 2 (NELL2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NEL Like Protein 2 (NELL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.115 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of NEL Like Protein 2 (NELL2). No significant cross-reactivity or interference between NEL Like Protein 2 (NELL2) and analogues was observed. | ||||||||||||||||||||||
Publications | NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growth. Cell Rep. 36, 109254 (2021) | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay | ||||||||||||||||||||||
Description |
Gene Symbol | NELL2 |
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Gene Full Name | neural EGFL like 2 |
Alias Symbols | NEL-like protein 2;NEL-related protein 2;neural epidermal growth factor-like 2;NRP2;protein kinase C-binding protein NELL2. |
NCBI Gene Id | 4753 |
Protein Name | Protein kinase C-binding protein NELL2 |
Description of Target | Required for neuron survival through the modulation of MAPK pathways (By similarity). Involved in the regulation of hypothalamic GNRH secretion and the control of puberty (By similarity). |
Uniprot ID | Q99435 |
Protein Accession # | NP_001138579 |
Nucleotide Accession # | NM_001145107.1 |
Protein Size (# AA) | 816 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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