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Datasheets/Manuals | Printable datasheet for anti-NELFB (ARP65310_P050) antibody |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human COBRA1 |
Purification | Affinity purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Yeast: 90% |
Peptide Sequence | Synthetic peptide located within the following region: NCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELRD |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-NELFB (ARP65310_P050) antibody is Catalog # AAP65310 |
Gene Symbol | NELFB |
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Gene Full Name | negative elongation factor complex member B |
Alias Symbols | COBRA1, NELF-B |
NCBI Gene Id | 25920 |
Protein Name | negative elongation factor B |
Description of Target | NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008] |
Uniprot ID | Q8WX92 |
Protein Accession # | NP_056271 |
Nucleotide Accession # | NM_015456.4 |
Protein Size (# AA) | 580 |
Molecular Weight | 66 kDa |
Protein Interactions | UBC; ABCF1; DIMT1; EDC4; IGF2BP3; SYNCRIP; EIF2B2; EIF2B3; EIF3C; YBX3; RPL23A; RFC4; PTBP1; PA2G4; YBX1; NMT1; MRE11A; ILF2; HNRNPU; FKBP3; DHX9; DDX1; KHDRBS3; DENR; YWHAB; MGST1; HSPA9; Dlg4; POLR2C; NELFB; SUPT5H; POLR2A; ATP1A1; ALDH3A2; NELFCD; CCT8 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "NELFB Antibody - N-terminal region (ARP65310_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "NELFB Antibody - N-terminal region (ARP65310_P050)"?
This item is available "Domestic: within 24 hours delivery | International: 3-5 days".
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What buffer format is "NELFB Antibody - N-terminal region (ARP65310_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "NELFB Antibody - N-terminal region (ARP65310_P050)"?
This target may also be called "COBRA1, NELF-B" in publications.
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What is the shipping cost for "NELFB Antibody - N-terminal region (ARP65310_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "NELFB Antibody - N-terminal region (ARP65310_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "NELFB Antibody - N-terminal region (ARP65310_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "66 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "NELFB Antibody - N-terminal region (ARP65310_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "NELFB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "NELFB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "NELFB"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "NELFB"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "NELFB"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "NELFB"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.