Catalog No: OKCD00614 (Formerly GWB-KBBWN4)
Size:96WELLS
Price: $750.00
SKU
OKCD00614
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Datasheets/ManualsPrintable datasheet for NEIL1 ELISA Kit (Human) (OKCD00614)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nei Endonuclease VIII Like Protein 1 (NEIL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nei Endonuclease VIII Like Protein 1 (NEIL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nei Endonuclease VIII Like Protein 1 (NEIL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nei Endonuclease VIII Like Protein 1 (NEIL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nei Endonuclease VIII Like Protein 1 (NEIL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nei Endonuclease VIII Like Protein 1 (NEIL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NEIL1 Microplate96 Wells (12 x 8 Well Strips)
NEIL1 Lyophilized Standard2 x 10 ng
100X Biotinylated NEIL1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.057 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Nei Endonuclease VIII Like Protein 1 (NEIL1). No significant cross-reactivity or interference between Nei Endonuclease VIII Like Protein 1 (NEIL1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolNEIL1
Gene Full Namenei like DNA glycosylase 1
Alias SymbolsDNA endonuclease eight-like glycosylase 1;DNA glycosylase/AP lyase Neil1;DNA-(apurinic or apyrimidinic site) lyase Neil1;endonuclease 8-like 1;endonuclease VIII;endonuclease VIII-like 1;FPG1;hFPG1;NEH1;nei endonuclease VIII-like 1;nei homolog 1;NEI1;nei-like protein 1.
NCBI Gene Id79661
Protein NameEndonuclease 8-like 1
Description of TargetInvolved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.
Uniprot IDQ96FI4
Protein Accession #NP_001243481
Nucleotide Accession #NM_001256552.1
Protein Size (# AA)390
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