NEFM Antibody (OAEE00503)

Catalog No: OAEE00503

Size:100UG

Western blotting analysis of neurofilament medium protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-09.

Immunofluorescence staining of neurofilament medium protein in murine Neuro2A cells by antibody NF-09conjugated with Dyomics 547 (red). DNA stained by Hoechst (blue). 

Product Info

• Predicted Species Reactivity: Mammal
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: NF-09
• Isotype: IgG2a
• Host: Mouse
• Application: Immunocytochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Pellet of porcine brain cold stable proteins after depolymerization of microtubules.
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody NF-09 reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Draberova E, Sulimenko V, Kukharskyy V, Draber P: Monoclonal antibody NF-09 specific for neurofilament protein NF-M. Folia Biol (Praha). 1999;45(4):163-5.
  Ohara O, Gahara Y, Miyake T, Teraoka H, Kitamura T:Neurofilament deficiency in quail caused by nonsense mutation in neurofilament-L gene. J Cell Biol. 1993 Apr;121(2):387-95.
  Rao MV, Campbell J, Yuan A, Kumar A, Gotow T, Uchiyama Y, Nixon RA: The neurofilament middle molecular mass subunit carboxyl-terminal tail domains isessential for the radial growth and cytoskeletal architecture of axons but notfor regulating neurofilament transport rate. J Cell Biol. 2003 Dec 8;163(5):1021-31.
  Deng Y, Li B, Liu F, Iqbal K, Grundke-Iqbal I, Brandt R, Gong CX: Regulation between O-GlcNAcylation and phosphorylation of neurofilament-M andtheir dysregulation in Alzheimer disease. FASEB J. 2008 Jan;22(1):138-45.

Target Info

• Gene Symbol: NEFM
• Gene Full Name: neurofilament medium chain
• Alias Symbols: 160 kDa neurofilament protein;NEF3;neurofilament 3;neurofilament medium polypeptide;neurofilament triplet M protein;neurofilament, medium polypeptide 150kDa;neurofilament-3 (150 kD medium);NFM;NF-M.
• NCBI Gene Id: 4741
• Protein Name: Neurofilament medium polypeptide
• Description of Target: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
• Uniprot ID: P07197
• Protein Accession #: NP_005373.2
• Nucleotide Accession #: NM_005382.2