Catalog No: OKCD02751
Size:96WELLS
Price: $700.00
SKU
OKCD02751
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Datasheets/ManualsPrintable datasheet for NEFH ELISA Kit (Mouse) (OKCD02751)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)85-99%78-101%78-92%79-90%
EDTA Plasma (n=5)89-98%82-96%91-102%81-97%
Heparin Plasma (n=5)95-105%78-89%95-103%87-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament, Heavy Polypeptide (NEFH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament, Heavy Polypeptide (NEFH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofilament, Heavy Polypeptide (NEFH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neurofilament, Heavy Polypeptide (NEFH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10484
EDTA Plasma (n=5)86-9489
Heparin Plasma(n=5)80-9687
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurofilament, Heavy Polypeptide (NEFH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurofilament, Heavy Polypeptide (NEFH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NEFH Microplate96 Wells (12 x 8 Well strips)
NEFH Lyophilized Standard2
100X Biotinylated NEFH Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity5.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Neurofilament, Heavy Polypeptide (NEFH). No significant cross-reactivity or interference between Neurofilament, Heavy Polypeptide (NEFH) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolNefh
Gene Full Nameneurofilament, heavy polypeptide
Alias Symbols200 kDa neurofilament protein;mKIAA0845;NEF;neurofilament 200kDa;neurofilament heavy polypeptide;neurofilament triplet H protein;NF-;NF20;NF200;Nfh;NF-H.
NCBI Gene Id380684
Protein NameNeurofilament heavy polypeptide
Description of TargetNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
Uniprot IDP19246
Protein Accession #NP_035034.2
Nucleotide Accession #NM_010904.3
Protein Size (# AA)1090
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