NEDD4L Antibody - middle region (ARP43192_P050)

Catalog No: ARP43192_P050

WB Suggested Anti-NEDD4L Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: PANC1 cell lysate

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NEDD4L
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: TVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLP
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-NEDD4L (ARP43192_P050) antibody is Catalog # AAP43192 (Previous Catalog # AAPP25161)
• Enhanced Validation:

  Relative Expression (Western Blot)

• Reference: Raikwar,N.S. Am. J. Physiol. Renal Physiol. 294 (5), F1157-F1165 (2008)

Target Info

• Gene Symbol: NEDD4L
• Gene Full Name: Neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
• Alias Symbols: RSP5, PVNH7, NEDD4-2, NEDD4.2, hNEDD4-2
• NCBI Gene Id: 23327
• Protein Name: E3 ubiquitin-protein ligase NEDD4-like
• Description of Target: NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
• Uniprot ID: Q96PU5-5
• Protein Accession #: NP_056092
• Nucleotide Accession #: NM_015277
• Protein Size (# AA): 955
• Molecular Weight: 110 kDa
• Protein Interactions: NEDD4L; DAZAP2; UBE2D1; PTCH1; WNK3; TNK2; SNCA; SLC9A3; SMAD6; KCNH2; HCN1; SMAD3; UBE2N; SNA3; NOTCH1; UBE2D3; UBC; SGK1; KCNQ1; SGK3; GORAB; MED28; FBXL15; vpr; RNF11; AATF; YWHAQ; LAPTM5; SCNN1B; SMAD2; ARRDC3; ARRDC4; UBE2L3; UBE2D2; SMAD7; UBE2K; UB