Catalog No: OKWB00256
Size:96T
Price: $450.00
SKU
OKWB00256
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Datasheets/ManualsPrintable datasheet for NECTIN2 ELISA Kit (Mouse) (OKWB00256)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-105%88-104%89-104%85-103%
EDTA Plasma (n=5)82-98%87-90%89-100%86-100%
Heparin Plasma (n=5)80-99%85-94%83-100%84-100%
ELISA Kit PrincipleAviva Systems Biology Nectin2 ELISA Kit (Mouse) (OKWB00256) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nectin2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nectin2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nectin2 captured in the well.
ELISA Kit Range78.125-5000pg/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)93-10398
EDTA Plasma (n=5)86-9391
Heparin Plasma (n=5)86-10494
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Nectin2 Microplate96 Wells (12 x 8 Well strips)
Nectin2 Lyophilized Standard2 x 5 ng
100X Nectin2 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity46.9 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolNectin2
Gene Full Namenectin cell adhesion molecule 2
Alias SymbolsAI325026;AI987993;Cd112;herpes virus entry mediator B;herpesvirus entry mediator B;hveB;mHveB;MP;MPH;murine herpes virus entry protein B;murine herpesvirus entry protein B;necti;Nectin cell adhesion molecule 2;nectin-2;P;poliovirus receptor homolog;poliovirus receptor-related 2;poliovirus receptor-related protein 2;poliovirus sensitivity;Pvr;Pvrl2;Pvs.
NCBI Gene Id19294
Protein NameNectin-2
Description of TargetModulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein.
Uniprot IDP32507
Protein Accession #NP_001153196
Nucleotide Accession #NM_001159724
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