Catalog No: OKWB00256
Size:96T
Price: $450.00
SKU
OKWB00256
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for NECTIN2 ELISA Kit (Mouse) (OKWB00256) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology Nectin2 ELISA Kit (Mouse) (OKWB00256) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nectin2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nectin2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nectin2 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 78.125-5000pg/ml | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 46.9 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Type: Quantitative Sandwich ELISA |
Gene Symbol | Nectin2 |
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Gene Full Name | nectin cell adhesion molecule 2 |
Alias Symbols | AI325026;AI987993;Cd112;herpes virus entry mediator B;herpesvirus entry mediator B;hveB;mHveB;MP;MPH;murine herpes virus entry protein B;murine herpesvirus entry protein B;necti;Nectin cell adhesion molecule 2;nectin-2;P;poliovirus receptor homolog;poliovirus receptor-related 2;poliovirus receptor-related protein 2;poliovirus sensitivity;Pvr;Pvrl2;Pvs. |
NCBI Gene Id | 19294 |
Protein Name | Nectin-2 |
Description of Target | Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein. |
Uniprot ID | P32507 |
Protein Accession # | NP_001153196 |
Nucleotide Accession # | NM_001159724 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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