Catalog No: OKEH01153
Size:96T
Price: $525.00
SKU
OKEH01153
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NDUFS7 ELISA Kit (Human) (OKEH01153) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NDUFS7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NDUFS7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NDUFS7 captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
NDUFS7 Microplate96 Wells (12 x 8 Well strips)
NDUFS7 Lyophilized Standard2
100X Biotinylated NDUFS7 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNDUFS7
Gene Full NameNADH:ubiquinone oxidoreductase core subunit S7
Alias SymbolsCI-20;CI-20KD;complex I 20kDa subunit;complex I, mitochondrial respiratory chain, 20-KD subunit;complex I-20kD;MC1DN3;MY017;NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20kDa (NADH-coenzyme Q reductase);NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial;NADH:ubiquinone oxidoreductase PSST subunit;NADH-coenzyme Q reductase;NADH-ubiquinone oxidoreductase 20 kDa subunit;PSST;PSST subunit.
NCBI Gene Id374291
Protein NameO75251
Description of TargetNADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
Uniprot IDhttps://www.uniprot.org/uniprot/O75251
Protein Accession #NP_077718.3
Nucleotide Accession #NM_024407.4
Protein Size (# AA)213
Molecular Weight24 kDa
Write Your Own Review
You're reviewing:NDUFS7 ELISA Kit (Human) (OKEH01153)
Your Rating
We found other products you might like!