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Catalog No: OKEH08038
Size:96T
Price: $800.00
SKU
OKEH08038
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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NDUFA6 ELISA Kit (Bovine) (OKEH08038)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology NDUFA6 ELISA Kit (Bovine) (OKEH08038) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NDUFA6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NDUFA6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NDUFA6 captured in well.
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=2.5% (n = 20)
Mean Inter-assay CV%: <=11.5% (n = 20)
ELISA Kit Component
ComponentAmount
NDUFA6 Microplate96 Wells (12 x 8 Well strips)
NDUFA6 Lyophilized Standard2
100X Biotinylated NDUFA6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
::Subcellular Location: Mitochondrion inner membrane Peripheral membrane protein Matrix side
::Unigene: Bt.63
SMR: Q02366
String: 9913.ENSBTAP00000012512
::KEGG: bta:327670
Pfam: PF05347
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Bovine NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNDUFA6
Gene Full NameNADH:ubiquinone oxidoreductase subunit A6
Alias SymbolsB14;CI-B14;complex I-B14;NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6, 14kDa;NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6;NADH-ubiquinone oxidoreductase B14 subunit.
NCBI Gene Id327670
Protein NameQ02366
Description of TargetNADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
Uniprot IDhttps://www.uniprot.org/uniprot/Q02366
Protein Accession #NP_786985.1
Nucleotide Accession #NM_175791.2
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