NDUFA10 Antibody (OAGA00214)

Catalog No: OAGA00214

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:H1299
B:Raji
10% SDS PAGE
OAGA00214 diluted at 1:500

Immunohistochemical analysis of paraffin-embedded colon, using NDUFA10(OAGA00214) antibody at 1:100 dilution.

NDUFA10 antibody [N1C3] detects NDUFA10 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: NDUFA10 protein stained by NDUFA10 antibody [N1C3] (OAGA00214) diluted at 1:500.
Blue: Hoechst 33342 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC, WB
• Additional Information: Background: The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji
  Cellular Localization: Mitochondrion matrix
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 43 and 355 of NDUFA10 (Uniprot ID#O95299)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.82 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: NDUFA10
• Gene Full Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
• Alias Symbols: CI-42k, CI-42KD, MC1DN22
• NCBI Gene Id: 4705
• Protein Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
• Uniprot ID: O95299
• Protein Accession #: NP_004535.1
• Molecular Weight: 41 kDa