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Datasheets/Manuals | Printable datasheet for NDRG1 Polyclonal Antibody (OABB00372) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Dog|Horse|Human|Rabbit |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Application | Immunohistochemistry|Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Protein NDRG1 is a protein that in humans is encoded by the NDRG1 gene. It is a member of the N-myc downregulated gene family which belong to the alpha/beta hydrolase superfamily. The NDRG1 gene is mapped to chromosome 8q24. It has got a deduced 394 amino acid protein with a molecular mass of 43kD. NDRG1 is expressed in the cytoplasm and basolateral membranes of gut lumen surface epithelial cells. The protein appears to play a vital role in growth arrest and cell differentiation. |
:: | Background: Protein NDRG1 is a protein that in humans is encoded by the NDRG1 gene. It is a member of the N-myc downregulated gene family which belong to the alpha/beta hydrolase superfamily. The NDRG1 gene is mapped to chromosome 8q24. It has got a deduced 394 amino acid protein with a molecular mass of 43kD. NDRG1 is expressed in the cytoplasm and basolateral membranes of gut lumen surface epithelial cells. The protein appears to play a vital role in growth arrest and cell differentiation. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat |
Reference | 1. van Belzen N, Dinjens WN, Diesveld MP, Groen NA, van der Made AC, Nozawa Y, Vlietstra R, Trapman J, Bosman FT (Sep 1997). "A novel gene which is up-regulated during colon epithelial cell differentiation and down-regulated in colorectal neoplasms". Lab Invest 77 (1): 85–92. PMID 9251681. 2. Kokame K, Kato H, Miyata T (Jan 1997). "Homocysteine-respondent genes in vascular endothelial cells identified by differential display analysis. GRP78/BiP and novel genes". J Biol Chem 271 (47): 29659–65. doi:10.1074/jbc.271.47.29659. PMID 8939898. 3. Zhang J, Chen S, Zhang W, Zhang J, Liu X, Shi H, Che H, Wang W, Li F, Yao L (Jun 2008). "Human differentiation-related gene NDRG1 is a Myc downstream-regulated gene that is repressed by Myc on the core promoter region". Gene 417 (1-2): 5–12. 4. "Entrez Gene: NDRG1 N-myc downstream regulated gene 1". |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Protein NDRG1(NDRG1) detection. Tested with WB, IHC-P in Human. |
Gene Symbol | NDRG1 |
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Gene Full Name | N-myc downstream regulated 1 |
Alias Symbols | CAP43;CMT4D;differentiation-related gene 1 protein;DRG1;DRG-1;GC4;HMSNL;NDR1;nickel-specific induction protein Cap43;NMSL;N-myc downstream-regulated gene 1 protein;protein NDRG1;protein regulated by oxygen-1;PROXY1;reducing agents and tunicamycin-responsive protein;RIT42;RTP;TARG1;TDD5. |
NCBI Gene Id | 10397 |
Protein Name | Protein NDRG1 |
Description of Target | Stress-responsive protein involved in hormone responses, cell growth, and differentiation. Acts as a tumor suppressor in many cell types. Necessary but not sufficient for p53/TP53-mediated caspase activation and apoptosis. Has a role in cell trafficking, notably of the Schwann cell, and is necessary for the maintenance and development of the peripheral nerve myelin sheath. Required for vesicular recycling of CDH1 and TF. May also function in lipid trafficking. Protects cells from spindle disruption damage. Functions in p53/TP53-dependent mitotic spindle checkpoint. Regulates microtubule dynamics and maintains euploidy. |
Uniprot ID | Q92597 |
Molecular Weight | 42835 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "NDRG1 Polyclonal Antibody (OABB00372)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Dog|Horse|Human|Rabbit".
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How long will it take to receive "NDRG1 Polyclonal Antibody (OABB00372)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "NDRG1 Polyclonal Antibody (OABB00372)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "NDRG1 Polyclonal Antibody (OABB00372)"?
This target may also be called "CAP43;CMT4D;differentiation-related gene 1 protein;DRG1;DRG-1;GC4;HMSNL;NDR1;nickel-specific induction protein Cap43;NMSL;N-myc downstream-regulated gene 1 protein;protein NDRG1;protein regulated by oxygen-1;PROXY1;reducing agents and tunicamycin-responsive protein;RIT42;RTP;TARG1;TDD5." in publications.
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What is the shipping cost for "NDRG1 Polyclonal Antibody (OABB00372)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "NDRG1 Polyclonal Antibody (OABB00372)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "NDRG1 Polyclonal Antibody (OABB00372)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "42835 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "NDRG1 Polyclonal Antibody (OABB00372)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "NDRG1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "NDRG1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "NDRG1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "NDRG1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "NDRG1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "NDRG1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.