Catalog No: OKCD09504
Size:96WELLS
Price: $750.00
SKU
OKCD09504
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Datasheets/ManualsPrintable datasheet for NDNF ELISA Kit (Human) (OKCD09504)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuron Derived Neurotrophic Factor (NDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuron Derived Neurotrophic Factor (NDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuron Derived Neurotrophic Factor (NDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neuron Derived Neurotrophic Factor (NDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuron Derived Neurotrophic Factor (NDNF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuron Derived Neurotrophic Factor (NDNF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
NDNF Microplate96 Wells (12 x 8 Well strips)
NDNF Lyophilized Standard2
100X NDNF HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 5.9pg/mL
Gene SymbolNDNF
Gene Full Nameneuron derived neurotrophic factor
Alias SymbolsC4orf31;fibronectin type-III domain-containing protein C4orf31;HH25;Neuron-derived neurotrophic factor;NORD;protein NDNF.
NCBI Gene Id79625
Protein NameProtein NDNF
Description of TargetPromotes matrix assembly and cell adhesiveness (By similarity). Promotes neuron migration, growth and survival as well as neurite outgrowth (PubMed:20969804). Promotes endothelial cell survival, vessel formation and plays an important role in the process of revascularization through NOS3-dependent mechanisms (PubMed:24706764).
Uniprot IDQ8TB73
Protein Accession #NP_078850.3
Nucleotide Accession #NM_024574.3
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