Catalog No: OPCA03216
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OPCA03216
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Datasheets/ManualsPrintable datasheet for ND75 Recombinant Protein (Fruit fly) (OPCA03216) (OPCA03216)
Product Info
Predicted Species ReactivityDrosophila melanogaster
Product FormatLiquid or Lyophilized powder
HostDrosophila melanogaster
Additional InformationRelevance: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized
Reconstitution and Storage-20°C or -80°C
Formulation20 mM Tris-HCl based buffer, pH 8.0
PurityGreater than 90% as determined by SDS-PAGE.
Peptide SequenceSAMVAQTPAKAPEKIEVFVDDIPVQVVPGTTVLQAAAQIGVEIPRFCYHERLAVAGNCRMCLVEVEKSPKPVAACAMPVMKGWRIKTNSDLTRKAREGVMEFLLMNHPLDCPICDQGGECDLQDQAMAFGSDRSRFTDINYTGKRAVEDKDIGPLVKTIMTRCIHCTRCVRFASEIAGVDDLGTTGRGNDMQIGTYVEKLFLTELSGNVIDLCPVGALTNKPYSFVARPWEIRKVSSIDVLDAVGSNIVVSTRTNEVLRILPRENEDVNEEWLADKSRFACDGLKRQR
Protein SequenceSAMVAQTPAKAPEKIEVFVDDIPVQVVPGTTVLQAAAQIGVEIPRFCYHERLAVAGNCRMCLVEVEKSPKPVAACAMPVMKGWRIKTNSDLTRKAREGVMEFLLMNHPLDCPICDQGGECDLQDQAMAFGSDRSRFTDINYTGKRAVEDKDIGPLVKTIMTRCIHCTRCVRFASEIAGVDDLGTTGRGNDMQIGTYVEKLFLTELSGNVIDLCPVGALTNKPYSFVARPWEIRKVSSIDVLDAVGSNIVVSTRTNEVLRILPRENEDVNEEWLADKSRFACDGLKRQR
Storage BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
SourceE.coli
Protein Range28-315 aa
TagN-terminal 6xHis-SUMO-tagged
ReferenceIdentification of nuclear genes encoding mitochondrial proteins: isolation of a collection of D. melanogaster cDNAs homologous to sequences in the Human Gene Index database.Caggese C., Ragone G., Perrini B., Moschetti R., de Pinto V., Caizzi R., Barsanti P.Mol. Gen. Genet. 261:64-70(1999)
Gene SymbolND-75
Gene Full NameNADH dehydrogenase (ubiquinone) 75 kDa subunit
Alias SymbolsCG2286;CG2286-PA;CG2286-PB;Complex I-75kD;Dmel\CG2286;Dmel_CG2286;FBpp0071128;NADH dehydrogenase (ubiquinone) 75 kDa subunit;NADH ubiquinone oxidoreductase 75 kDa;NADH:ubiquinone oxidoreductase 75 kDa subunit;NADH:ubiquinone reductase 75 kDa subunit;NADH:ubiquinone reductase 75kD subunit precursor;NADH:ubiquinone reductase 75-kD subunit precursor;NADH-ubiquinone oxidoreductase 75 kDa subunit;ND75;ND-75-PA;ND-75-PB;NDUFS1;NDUS1;NUAM.
NCBI Gene Id31762
Protein NameNADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
Description of TargetCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).
Uniprot IDQ94511
Protein Accession #NP_511083.1
Nucleotide Accession #NM_078528.3
Protein Size (# AA)Recombinant
Molecular Weight47.8 kDa
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