Catalog No: OPCA03216
Price: $0.00
SKU
OPCA03216
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for ND75 Recombinant Protein (Fruit fly) (OPCA03216) (OPCA03216) |
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Predicted Species Reactivity | Drosophila melanogaster |
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Product Format | Liquid or Lyophilized powder |
Host | Drosophila melanogaster |
Additional Information | Relevance: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized |
Reconstitution and Storage | -20°C or -80°C |
Formulation | 20 mM Tris-HCl based buffer, pH 8.0 |
Purity | Greater than 90% as determined by SDS-PAGE. |
Peptide Sequence | SAMVAQTPAKAPEKIEVFVDDIPVQVVPGTTVLQAAAQIGVEIPRFCYHERLAVAGNCRMCLVEVEKSPKPVAACAMPVMKGWRIKTNSDLTRKAREGVMEFLLMNHPLDCPICDQGGECDLQDQAMAFGSDRSRFTDINYTGKRAVEDKDIGPLVKTIMTRCIHCTRCVRFASEIAGVDDLGTTGRGNDMQIGTYVEKLFLTELSGNVIDLCPVGALTNKPYSFVARPWEIRKVSSIDVLDAVGSNIVVSTRTNEVLRILPRENEDVNEEWLADKSRFACDGLKRQR |
Protein Sequence | SAMVAQTPAKAPEKIEVFVDDIPVQVVPGTTVLQAAAQIGVEIPRFCYHERLAVAGNCRMCLVEVEKSPKPVAACAMPVMKGWRIKTNSDLTRKAREGVMEFLLMNHPLDCPICDQGGECDLQDQAMAFGSDRSRFTDINYTGKRAVEDKDIGPLVKTIMTRCIHCTRCVRFASEIAGVDDLGTTGRGNDMQIGTYVEKLFLTELSGNVIDLCPVGALTNKPYSFVARPWEIRKVSSIDVLDAVGSNIVVSTRTNEVLRILPRENEDVNEEWLADKSRFACDGLKRQR |
Storage Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Source | E.coli |
Protein Range | 28-315 aa |
Tag | N-terminal 6xHis-SUMO-tagged |
Reference | Identification of nuclear genes encoding mitochondrial proteins: isolation of a collection of D. melanogaster cDNAs homologous to sequences in the Human Gene Index database.Caggese C., Ragone G., Perrini B., Moschetti R., de Pinto V., Caizzi R., Barsanti P.Mol. Gen. Genet. 261:64-70(1999) |
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Gene Symbol | ND-75 |
Gene Full Name | NADH dehydrogenase (ubiquinone) 75 kDa subunit |
Alias Symbols | CG2286;CG2286-PA;CG2286-PB;Complex I-75kD;Dmel\CG2286;Dmel_CG2286;FBpp0071128;NADH dehydrogenase (ubiquinone) 75 kDa subunit;NADH ubiquinone oxidoreductase 75 kDa;NADH:ubiquinone oxidoreductase 75 kDa subunit;NADH:ubiquinone reductase 75 kDa subunit;NADH:ubiquinone reductase 75kD subunit precursor;NADH:ubiquinone reductase 75-kD subunit precursor;NADH-ubiquinone oxidoreductase 75 kDa subunit;ND75;ND-75-PA;ND-75-PB;NDUFS1;NDUS1;NUAM. |
NCBI Gene Id | 31762 |
Protein Name | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial |
Description of Target | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity). |
Uniprot ID | Q94511 |
Protein Accession # | NP_511083.1 |
Nucleotide Accession # | NM_078528.3 |
Protein Size (# AA) | Recombinant |
Molecular Weight | 47.8 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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