ND5 ELISA Kit (Pig) (OKEH08462)

Catalog No: OKEH08462

Size:96T

Pig ND5 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	105-115% (n = 20)	107-117% (n = 20)	107-120% (n = 20)	105-115% (n = 20)
  EDTA plasma(n=5)	109-118% (n = 20)	106-115% (n = 20)	98-108% (n = 20)	83-96% (n = 20)
  heparin plasma(n=5)	109-119% (n = 20)	97-107% (n = 20)	105-115% (n = 20)	105-116% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology ND5 ELISA Kit (Pig) (OKEH08462) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ND5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ND5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ND5 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.0% (n = 20)
  Mean Inter-assay CV%: <=7.5% (n = 20)
• ELISA Kit Component:
  • ND5 Microplate: 96 Wells (12 x 8 Well strips)
  • ND5 Lyophilized Standard: 2
  • 100X Biotinylated ND5 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  Subcellular Location: Mitochondrion inner membrane Multi-pass membrane protein
  String: 9823.ENSSSCP00000019145
  KEGG: ssc:808511
  Pfam: PF06455
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.059ng/mL
• Specificity: Natural and recombinant Pig NADH-ubiquinone oxidoreductase chain 5
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ND5
• Gene Full Name: NADH dehydrogenase subunit 5
• Alias Symbols: KEF22_p03;NADH dehydrogenase subunit 5.
• NCBI Gene Id: 808511
• Protein Name: Q9TDR1
• Description of Target: NADH-ubiquinone oxidoreductase chain 5
• Uniprot ID: https://www.uniprot.org/uniprot/Q9TDR1
• Protein Accession #: NP_008644.1
• Nucleotide Accession #: NC_000845.1