Catalog No: OKEH07720
Size:96T
Price: $725.00
SKU
OKEH07720
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

NCOA3 ELISA Kit (Human) (OKEH07720)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology NCOA3 ELISA Kit (Human) (OKEH07720) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NCOA3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NCOA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NCOA3 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 112% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.4% (n = 20)
Mean Inter-assay CV%: <=5.9% (n = 20)
ELISA Kit Component
ComponentAmount
NCOA3 Microplate96 Wells (12 x 8 Well strips)
NCOA3 Lyophilized Standard2
100X Biotinylated NCOA3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
::Subcellular Location: Cytoplasm Nucleus Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
::Unigene: Hs.592142
SMR: Q9Y6Q9
String: 9606.ENSP00000361066
::KEGG: hsa:8202
MIM: 601937
Pfam: PF07469
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Human Nuclear receptor coactivator 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNCOA3
Gene Full Namenuclear receptor coactivator 3
Alias SymbolsACTR;AIB1;AIB-1;amplified in breast cancer 1 protein;bHLHe42;CAGH16;CBP-interacting protein;class E basic helix-loop-helix protein 42;CTG26;KAT13B;nuclear receptor coactivator 3;pCIP;RAC3;receptor-associated coactivator 3;SRC3;SRC-3;steroid receptor coactivator protein 3;thyroid hormone receptor activator molecule 1;TNRC14;TNRC16;TRAM-1.
NCBI Gene Id8202
Protein NameQ9Y6Q9
Description of TargetNuclear receptor coactivator 3
Uniprot IDhttps://www.uniprot.org/uniprot/Q9Y6Q9
Protein Accession #NP_001167559.1
Nucleotide Accession #NM_001174088.1
Write Your Own Review
You're reviewing:NCOA3 ELISA Kit (Human) (OKEH07720)
Your Rating